Doubts and Suggestions 
EVIDENCE FOR SIMULTANEOUS 1Na+ :1Mg2+ AND PING PONG 2Na+ :1Mg2+ EXCHANGERS IN RAT THYMOCYTES
| dc.contributor.author | Contreras Jurado, Silvia Constanza | |
| dc.contributor.author | Sánchez Morito, Nuria | |
| dc.contributor.author | Ruiz Contreras, Anabel | |
| dc.contributor.author | González Martínez, Marco T. | |
| dc.contributor.author | Soler Diaz, Agatángelo | |
| dc.date.accessioned | 2021-11-10T17:11:22Z | |
| dc.date.available | 2021-11-10T17:11:22Z | |
| dc.date.created | 2005-05-01 | |
| dc.description.tableofcontents | Rat thymocytes showed two Na+ /Mg2+ exchangers with high- and low- affinities for external Na+ (Na+ o) at physiological internal Mg2+content. The total internal Mg2+ content (Mg2+it) was enhanced by loading with MgCl2 and the ionophore A-23187. Under these conditions, Na+ /Mg2+ exchangers were dramatically stimulated by the Mg2+it increase. Na+ -induced Mg2+ effluxes were independent of Cl o or H+ . The Na+ /Mg2+ exchangers, which we named HANao (high affinity for Na+ o) and LANao (low affinity for Na+ o), were dissected in Mg2+-loaded thymocytes according to their kinetics and stoichiometries. HANao, which showed an apparent dissociation constant for Na+ o (KNa H) = 9.2 +/- 1.6 mmol l -1 Na+ o and a maximal Na+ influx rate (VNa(Na H)max) = 30.5 +/- 6.1 mmol (l cells)-1 h-1, was a 1Na+ :1Mg2+ simultaneous antiporter insensitive to external magnesium (Mg2+o) whereas that LANao, with KNa L = 65.1 +/- 8.6 mmol l-1 Na+ and a VNa(Na L)max = 79.5 +/- 14.3 mmol (l cells)-1 Na+ h-1, was a 2Na+ :1Mg2+ ¿ping-pong¿ antiporter which was strongly inhibited by Mg2+o. At physiological concentration of Mg2+o (1 mM), the Na+ /Mg2+ exchange through the LANao was inhibited by ~50%. Amiloride (10-4 M) inhibited at similar extent both Na+ and Mg2+fluxes at high and at low Na+ o. | es_ES |
| dc.format | application/pdf | es_ES |
| dc.identifier.location | N/A | es_ES |
| dc.identifier.uri | https://hdl.handle.net/20.500.12080/26189 | |
| dc.language | eng | es_ES |
| dc.rights | CC-BY | es_ES |
| dc.rights.accessrights | info:eu-repo/semantics/openAccess | es_ES |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.es | es_ES |
| dc.title | EVIDENCE FOR SIMULTANEOUS 1Na+ :1Mg2+ AND PING PONG 2Na+ :1Mg2+ EXCHANGERS IN RAT THYMOCYTES | es_ES |
| dc.type | info:eu-repo/semantics/article | es_ES |